Archive for Bodybuilding Peptides

Bodybuilding with Mechano growth factor

MGF-mechano-growth-factor

Bodybuilding with Mechano growth factor

MGF (mechano growth factor) has been used by bodybuilders and athletes for a few years already. After resistance exercise, IGF-1 is released within the muscle. Specifically, at this time, immediately following the mechanical use of a muscle, the IGF-I gene is spliced towards MGF which initiates hypertrophy and repair of local muscle damage. It does so both by activating muscle stem cells and satellite cells, but also via various other anabolic processes. (1) It differs from “regular” IGF-1 mainly due to it’s C-Terminal sequence.

MECHANO GROWTH FACTOR Background

It was first discovered in the muscle by Goldspink, et al. In human muscle, a 49-base insert changes the reading frame in mechano growth factor (MGF) as compared to IGF-1.

What does MGF do?

When mechanical overload is introduced to a muscle (as by weight training), the IGF-1 gene released and is differentially spliced during the bodies response. Initially, it it is spliced to produce predominantly IGF-1Ec (called the MGF splice variant of IGF-1). This early splicing stimulates satellite cells into activation. Which in turn allows the activation of extra undamaged nuclei to grow new muscle fiber and tissue. The appearance of MGF also initiates the upregulation of new protein synthesis. After this initial splicing of IGF-1 into MGF, production then switches towards producing a systemic release of IGF-1Ea from the liver, which also upregulates protein synthesis as well. The expression of IGF-1 splice variants, over the course of the healing and regrowth phase of muscle repair is thought to be the primary anabolic mechanism by which the body produces new muscle. MGF is available as an injectable peptide, and it has been anecdotally shown that injecting it will cause a response in the area resulting in localized muscle growth.

From Studies done on MGF

In a rodent study, a single intramuscular injection into muscle resulted in a 25% increase in mean muscle fibre cross section area within three weeks. Using a similar protocol, liver-derived IGF-1 took four months to produce a 15% increase.

It would also appear that with regards to age, the young have a better ability to respond to MGF (4), and that the elderly experience a decreased response to MGF which results in a decreased ability to stimulate the growth of new muscle tissue.

Peptide GHRP-2 for bodybuilding

Peptide, GHRP-2, bodybuilding, CJC-1295

GHRP-2 for bodybuilding

The effects of GHRP-2 are GH secretion stimulation, appetite promotion, fat mass decrease and muscle mass increase, lowering of cholesterol level, skin and bones state improving, defense of the liver and anti-inflammatory action.

DOSE USED WITH GHRP-2:

The dose should be 300 mgs each day. These cjc1295 doses should be injected 3 times a day. Starting with one injection in the morning . The injections are usually made subcutaneously in an abdominal area. The time between injections should be atleast 3-4 hours (in order the body has the time to produce GH for further release). Do not consume food for between 15-30 minutes after your dosage. Best time is around 20-25 minute mark. GH pulses should peak within about 10 minutes after dosage. Fats and Carbohydrates affect the pulse dramatically. Protein has no effect on pulse. However, you can have a pure protein source in your stomach at anytime if choose to do so.

How to take GHRP-2 during a Cycle:

Each day would consist of
100mcg of CJC-1295
+
100-200 mcg GHRP-2
three times a day.
at
in the morning on an empty stomach 25 minutes before eating
in the afternoon – PWO
finally just before bed

Dilution or reconstitution of GHRP-2 :

GHRP-2 is a peptide which is bought in powdered form. In order to inject this compound, it must be reconstituted through the use of either sterile water or bacteriostatic water. Both are acceptable re-constitutional ingredients, however the use of sterile water will see the GHRP-2 degrade at a faster rate than bacteriostatic water. Mixing (reconstitution) the lyophilized product in their vials with Bacteriostatic Water (BW) can take some getting used to. The idea is not to add too much dilution. The typical rule of thumb is to add 0.5mL of BW to 1mg of Peptide. So a 2mg vial should reconstitute with 1mL BW. 5mg with 2.5mL, 10mg with 5mL, etc. Squirt the BW along the inside wall of the vial in a smooth controlled manner being cautious not to agitate the mixture too much. It will dissolve itself and become clear. You can roll the vial gently between your fingers or hands but don’t shake it to dissolve. The reconstitute is ok to be drawn once fully dissolved.

Melanotan II erections

peptide-vial

Melanotan II erections

a study done on it

Synthetic Melanotan II  peptide initiates erections in men with psychogenic erectile dysfunction: double-blind, placebo controlled crossover study.

PURPOSE Of the Melanotan II study :

We evaluated the erotogenic properties of a new cyclic alpha-melanocyte-stimulating hormone analogue, Melanotan-II, to treat men with psychogenic erectile dysfunction.
MATERIALS AND METHODS:

Ten men with erectile dysfunction of no known organic cause were entered in a double-blind, placebo controlled crossover study in which the erotogenic properties of Melanotan-II and a vehicle placebo were compared using real-time RigiScan monitoring. The presence, duration and rigidity of erections were recorded during a 6-hour period.
RESULTS:

In 8 of 10 men treated with Melanotan-II clinically apparent erections developed. Mean duration of tip rigidity greater than 80% was 38.0 minutes with Melanotan-II and 3.0 with placebo (p=0.0045). Transient side effects of nausea, stretching and yawning, and decreased appetite were reported more frequently after injections of Melanotan-II than placebo but none required treatment.

Findings of the Melanotan II study :

Melanotan-II is a potent initiator of erections in men with psychogenic erectile dysfunction and has manageable side effects at a dose of 0.025 mg./kg.

Beginner’s Guide To IGF1-lr3

Beginner’s Guide To IGF1-lr3

  • IGF-1 Reconstitution
  • Making 0.6% Acetic Acid from Vinegar
  • Injection Technique
  • Sterile Procedure
  • Items You Will Need
  • …and more!

Preface
The goal of this guide is to help both those that have not used IGF-1lr3 before and for those that simply would like a methodical approach to the “mechanics” of running it.  This guide does not expand on the biochemistry of IGF-1, aside from a very simple introduction to it.  I suggest reading a book or searching forums to educate yourself about the biochemistry of “peptides” or “IGF” if you require in-depth knowledge.

I am not a physician, thus cannot and do not diagnose ailments or diseases and/or nor do I suggest that IGF-1 is a remedy for any illness or diseases.  IGF-1 should be treated with much respect.  It is research compound, thus you should use at your own risk.

Currently (05/31/2008), in the United States, IGF-1lr3 is a research compound.  It is legal to own this substance to the best of my knowledge (at current time).  I am not an attorney, so please review your local law(s) regarding possession and administration of this therapeutic protein.

I do not condone the usage of IGF-1lr3 unless you are qualified to do so.  This guide is provided as a research & development tool only.

IGF-1lr3 Overivew

Background:
Long Arg3 Insulin-like Growth Factor-I (Long-R3-IGF-I) is an 83 amino acid analog of IGF-I comprising the complete IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Long-R3-IGF-I is significantly more potent than IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.

Description:
Recombinant Human Long-R3-IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9111 Dalton.

0.6% Acetic Acid Overview
Acetic Acid (AA) will be used to reconstitute (turn your lyophilized IGF-1 into a liquid form) your IGF-1.  The standard is to use 0.6% AA.  This concentration is typically not available for you to purchase.  You can make your own 0.6% AA and I will show you  how below (many have used this method successfully).

Making 0.6% Acetic Acid
You will have to purchase a few items upfront.  Here is a “grocery list” of items you will need.  I have provided check boxes for you to check off once you have purchased these items.

Items Needed:
• Distilled white vinegar (grocery store)
• Distilled water (grocery store)
• 0.2-0.22um sterile Whatman syringe filter
• 10mL syringe with a luer lock tip
• ~20-22 gauge needles (just the needles)
• Sterile glass vial (10-20mL)
• Alcohol prep pads – sterile kind (70% isopropyl alcohol)




Quick Guide:
1. Swab the top of your sterile vial with alcohol prep pad (70% isopropyl alcohol)
2. Mix 7.5mL distilled water with 1.0mL vinegar
3. Add Whatman syringe filter
4. Add sterile ~20ga. needle to end of  Whatman filter
5. Inject the 8.5mL of solution into the sterile vial
6. You now have sterile 0.6% acetic acid

Detailed Directions:
1. Wash you hands thoroughly
2. Optional: wear alcohol treated exam gloves (rub your gloved hands together with 70% isopropyl alcohol on them until dry)
3. Using a sterile alcohol prep pad, swab the top of your sterile glass vial (into which the acetic acid solution will be held in)
4. Using  the 10mL syringe with a ~20ga. needle on the end, draw up 7.5mL distilled water
5. Using the same syringe, now draw up 1mL vinegar
6. Remove needle from the syringe and discard
7. Attach 0.2-0.22um Whatman sterile syringe filter (do not touch the free end that will have a needle on it)
8. Put a new, sterile needle (~20 gauge) onto the free end of the Whatman filter (do not touch needle)
a. Do not use the same needle on the Whatman that was used to originally draw up the unsterile vinegar and distilled water.
9. Put a ~20 gauge sterile needle into the top of your sterile glass vial to act as a vent
10. Inject the acetic acid solution into the vial
11. You are now done and should have sterile 0.6% acetic acid

Notes:
1. These items MUST be sterile:  20-22ga. Needles, whatman filter, glass vial
2. Whatman filter:  These small, sterile filters are used to filter the acetic acid solution so it is sterile.  It does not matter that the liquid in your syringe (distilled water & vinegar) is not sterile, nor does it matter that the syringe itself is not sterile.  Once the liquid goes through the filter it is STERILE.  Thus, everything after the filter must be sterile!
3. You will most likely use 1mL (milliliter) of 0.6% AA to reconstitute your IGF-1.  Thus, you should make at least 1.5mL.  In reality, it’s just as easy to make 8.5mL as I have stated in the above directions.  You will have plenty for use later then.
4. Do NOT reuse the Whatman filter nor any needles!  Discard immediately.

Reconstituting IGF-1lr3
Reconstitution is simply the addition of the 0.6% AA to your lyophilized IGF-1.
Assumption:  1mg/mL IGF-1/AA (1mg IGF-1 will be combined with 1mL AA; 1mg IGF-1 is the same as 1,000mcg)

1. Swab the top of your IGF-1 vial with a sterile alcohol prep pad
2. Swab the top of your 0.6% AA vial with a sterile alcohol prep pad
3. Using either multiple insulin syringe volumes (example: 2 x 0.5cc) or a single large syringe, obtain 1.0mL of 0.6% AA.
4. In the IGF-1 vial, insert a sterile ~20 ga. needle to act as a vent
5. Inject the 1.0mL of AA very slowly and dribble it down the side of the vial.
a. Be very careful with this peptide as it is very delicate!
6. Remove the needle & syringe and discard
7. Gently swirl the vial or roll between your hands.
a. Again, be very gentle here
8. You now have 1mg/mL of IGF-1
a. This is the same as:  1,000mcg/mL

Notes:
1. If you added 2mL of AA, it would be a 0.5mg/mL

Injecting IGF-1lr3
If this is your first time with injections, don’t worry.  You will be using a very fine gauge insulin syringe which means you will most likely have nearly effortless injections.  These things are so tiny and sharp you may not even feel it penetrating.  If you use sterile procedure, aspirate prior to injection, and have diluted your IGF-1/AA solution with enough bacteriostatic water (BW), you should have no issues with your injections and very minimal post-injection discomfort (if any at all!).

I cannot stress enough the importance on two topics:  A)  sterility, and B) pre-injection aspiration.  Always swab the injection site(s) with a sterile isopropyl alcohol (IPA) pad and aspirate prior to injecting the IGF-1.  No questions asked!

You will most likely intramuscular (IM) injections, but subcutaneous (sub-q) injections are also followed by some, but current theory is that IM will yield a localized effect.  By “localized effect”, I am referring to the effect IGF-1 will have at the injection site.  So if you inject IM into biceps, it is thought that your bicep muscles will get more of a dose of IGF-1 than other parts of your body (some which you don’t want to be effected, such as the intestines).  Both types of injections will have systemic effects (affecting the body as a whole).  Long R3 IGF-1 has an estimated half-life of 20-30hrs (taken from IGTROPIN data).

This guide assumes you will be doing bilateral IM injections. More below.

Bilateral injections are injections that are evenly divided between two muscles.  If you are injecting 40mcg (micrograms) bilaterally, you will be injecting 20mcg into the right bicep and 20mcg into the left bicep.

Current theorized best practice is to you inject your peptide post workout (PWO).  You have a small window of optimal opportunity.  Ideally, you would inject immediately PWO, but some do not like the idea of injecting in a public location, such as the gym.  Your next best option is to make your way home ASAP and have your needles loaded and ready (with your alcohol swabs sitting near by).

Sterility
Without a doubt, sterility is a major concern with injections.  You have to be conscious of bacteria and other infectious agents at all times when performing injections or other procedures that require sterility (such as reconstitutions and making 0.6% AA).

Bacteria (and viruses, and spores, etc) are invisible to the naked eye.  Yet they are everywhere. It is very important that you acquire sterile alcohol prep pads (make sure it says “sterile” before you buy them).  They are extremely cheap and effective.

Wash your hands! Before attempting anything requiring sterile technique, wash your hands and dry them with a clean paper towel (not the dirty towel hanging in the bathroom!).  For optimal sterility, you may purchase exam gloves (latex or non-latex) and, after putting them on, you can dump some isopropyl alcohol (IPA) onto them and rub your hands together thoroughly.  Now you really have sterile hands.  Exam gloves are very inexpensive as is the bottle of IPA.  IPA can be purchased for ~$1/bottle in the grocery store where the band-aids and whatnot.

I recommend you use a fresh syringe for each injection.
  Yes, some choose to use one syringe, but my feeling is that the syringes are so inexpensive and the risk of cross-contamination from one injection site to the other isn’t worth the risk.  Furthermore, every time your syringe needle has to penetrate something (rubber stoppers in vials, skin, etc) it dulls the tip.  Thus, maximum comfort is also achieved with fresh syringes.

This topic of “one or two syringes” can be argued, but if it’s your first time, play it safe and get off to a great start by using 2!

Pre-injection Aspiration 
Pre-injection aspiration is what you do after the needle has penetrated the muscle.  You must gently and slightly pull back on the needle’s plunger to see if you have hit a vein/artery.

Either of two things will happen upon aspiration:  A) bubbles/air and/or clear liquid will appear in the syringe (this is good), or B) blood will appear (bad).

If A) occurs, proceed with your injection. If B) occurs, then simply withdraw the needle, and re-pin a different location in that same muscle.  You do NOT want to inject your solution into a vein/artery!  This may result in very serious consequences.  Don’t worry, you can avoid this by simply aspirating slightly.  Have faith in yourself.

Injection Procedure
First, do not get all worked up over injecting IGF-1.  Easier said than done, I know.  But the reality is, the insulin syringes are extremely gentle.  Also, millions of people around the world, including women and children, use these syringes daily to treat Diabetes.  So you know it can’t be that bad (seriously)!  I highly recommend watching a couple videos on youtube regarding intramuscular (IM) injections to get a general idea of how they’re done if you’ve never witnessed them!

Back-Loading With Bacteriostatic Water (BW)
Back-loading is a process in which you dilute the IGF-1/AA solution that is in your syringe. The point is to dilute the acidity to a point that it will no longer cause tissue necrosis (death/damage) or pain upon injection.  It is recommended to dilute no less than 4:1 (4 parts BW to 1 part IGF-1/AA).

Example:  If you are injecting 40mcg bilat, IM, you will have two syringes each with 20mcg IGF-1.  Assume you want to draw 2 IU IGF-1. You will draw 2 IUs of the IGF-1/AA solution, then draw 2×4 = 8 IUs of BW (four times the amount of IGF-1/AA solution).  The total number of IUs in each syringe will be 2 + 8 = 10 IUs.  It will not hurt you if you decide to back-load with more BW.  It is a personal preference.

***Use my Excel-based “IGF-1” calculator to determine how many IUs you will need for a particular insulin syringe (1cc, 0.5cc, 0.3cc).

Recommended Best Injection Method:  Injecting bilaterally, post workout, intramuscularly (Bilat, PWO, IM)




Items you will need
1. Alcohol prep pads
2. 2 insulin syringes
3. Bacteriostatic water (BW)
4. Optional:  exam gloves
5. Optional:  IPA (to rub gloves with and to clean the surrounding area)

Injection Directions
1. Wash your hands thoroughly
2. Optional: put on exam gloves and rub with IPA until dry
3. Using an alcohol swab, clean the tops of both the IGF-1 vial and the BW vial.
4. Using a fresh alcohol swab, thoroughly clean the injection sites (let dry)
5. Fill each syringe with the appropriate amount of IGF-1/AA solution
a. Do NOT touch the needles to anything but sterile surfaces!
b. It is recommended that you clean/sanitize the area/surfaces you’re working in, in case you mindlessly touch a needle to a table (or other area).
6. Back-loading:  Draw up the necessary amount of BW into each syringe.
a. Tilt the needle up and down so the bubble(s) rise and fall, which mixes the solution slightly
7. With the needle pointing up, flick the syringe body to get the bubbles to rise to the needle
8. Slowly expel the air; be careful to not quirt liquid out as this wastes IGF-1
a. It takes >3mL of air to cause harm; small volumes of accidentally injected air will most likely be absorbed by muscle tissue
9. Insert syringe and aspirate by slightly pulling up on the plunger to see if you have hit a vessel. If you see blood, remove needle, and try again (no need to change syringes).  If you do NOT see blood, proceed to inject.
10. Perform “7.” thru “9” above on other side.
11. Discard sharps in appropriate container



Glossary

Acetic Acid (AA):  An acid that, when diluted to 0.6%, will act as a preservative for your IGF-1.  An off-the-shelf version of 5% AA is distilled white vinegar; your IGF-1 may be supplied in acetic acid (usually 0.6%)

Aspiration:  The technique of checking to see if your inserted needle is in a blood vessel.  It is performed by gently pulling up on the syringe plunger until you either see bubbles/air/clear liquid, or blood.  If you see blood, remove needle, and re-try the insertion.

Back-loading:  The process of diluting your IGF-1/AA with bacteriostatic water, prior to injection.  The purpose is to dilute the acidity of the AA so it doesn’t cause tissue damage and so it doesn’t cause injection burn/discomfort.
A. Draw desired amount of IGF-1/AA solution
B. Back-load with BW:  draw desired amount of BW

Bacteriostatic Water (BW)
:  This is water for injection (sterile) that has benzoyl alcohol (BA) added to it to ward of contamination.  You use BW to dilute your IGF-1/AA solution prior to injection (aka, “back-loading”).

Bilateral Injection (bilat):  An injection which involves the administration of IGF-1 in equal amounts to each side of the body.  If you are injecting 40mcg IGF-1 into the biceps bilaterally, you will be injecting 20mcg into each bicep (left & right side).

Distilled Water:  Has virtually all of its impurities removed through distillation. Distillation involves boiling the water and then condensing the steam into a clean cup, leaving nearly all of the solid contaminants behind. This is NOT sterile water.  It can be purchased in any grocery store in the “water” isle.

Endogenous:  Substances that originate from within an organism, tissue, or cell.  It is the opposite of exogenous

Exogenous:  Refers to an action or object coming from outside a system.  It is the opposite of endogenous.

IM: Intramuscular; typically refers to the type of injection where you inject a substance directly into muscle tissue

IGF-1 lr3:  A peptide that is responsible for new muscle tissue development; it is synthetic and has a much longer circulatory life than endogenous IGF-1

Lyophilized:  The form in which IGF-1 is typically supplied; this is a freeze-dried protein which is performed in a vacuum; appearance may range from a fine, loose white powder, to a white solid “paste”-type substance

PWO:  Post Work Out; refers to the time period when the administration of IGF-1 is thought to be the most effective (immediately PWO).

Reconstitution:  The addition of 0.6% acetic acid to lyophilized IGF-1r3 to get it into solution.  Typically one reconstitutes using 1mL or 2mL of acetic acid, yielding 1mg/mL or 2mg/mL of IGF-1/AA.

Sub-q:  Subcutaneous; typically refers to the type of injection where you inject a substance under the skin; this results in systemic distribution of substances

Author: PapaPumpSD


Reconstituting HGH

Reconstituting HGH the numbers breakdown

For an 10iu vial of HGH (human growth hormone), Jintropin and Generic Chinese

you add 1 ml (is=to 100iu’s on a slin pin) of bacteriostatic water or the sterile solution to your vial of HGH. . You will need to provide on some kits (chinese)  On an insulin needle 1iu of reconstituted gh is 10iu’s on the pin, so if your using 2iu’s of gh ed.. it would be 20iu’s on the insulin needle 3iu’s ed  it would be 30iu’s on the insulin needle 4iu’s ed  it would be 40iu’s on the insulin needle 5iu’s ed  it would be 50iu’s on the insulin needle 6iu’s ed  it would be 60iu’s on the insulin needle

 

For an 18iu vial of gh

you add 1 ml (1ml is equal to 100iu’s on a insulin needle) of bacteriostatic water or the solution they provide.  on a slin pin 1iu of reconstituted gh is 5.5iu’s on the pin  so if your doing  2iu’s of gh ed.. it would be 11iu’s on the insulin needle 3iu’s ed.. it would be 16.5iu’s on the slin pin 4iu’s ed.. it would be 22iu’s on the slin pin 5iu’s ed.. it would be 27.5iu’s on the slin pin 6iu’s ed.. it would be 33iu’s on the slin pin

21iu’s of active GH per vial

The fact is that we have 21iu’s of gh in each vial… Now we also have up to 1.6mls of bacteriostatic water to use.. It’s not necessary that we use it all..
We need to come up with a nice round number that would be easy to read and remember.  OK If we use 105iu’s of water to mix with the GH than we have a ratio of 1:5 1iu of Gh per 5 iu’s of water.
So if your doing  2iu’s of gh ed.. then thatwould be 10 on an insulin needle 3iu’s ed.. then thatw ould be 15 on a slin pin 4iu’s ed.. then that would be 20 on a slin pin 5iu’s ed.. then that would be 25 on a slin pin; 6iu’s ed.. then that would be 30 on a slin pin

 

and so on

You need to know how many iu’s your hgh is in powder form (before Reconstituting)

Complete step-by-step guide for peptide beginners

Peptide Guide

1 – You are on this site because you have heard of and want to become more familiar with Growth Hormone Releasing Peptide (GHRP) and/or Growth Hormone Releasing Hormone (GHRH). These 2 materials administered can give you an increased quality of life in ways of anti-aging, muscular hypertrophy, fat loss, injury repair, higher bone density, and better sleep.

2 – GHRP can be used on its own to increase our natural Growth Hormone (GH) pulse release from the Pituitary Gland in the brain. GHRP dosed in conjunction with GHRH will amplify our growth hormone release significantly to gain maximal benefit.

3 – There are various types of GHRH’s. The only GHRH to consider is tetra-substituted CJC-1295 / CJC-1295(without DAC) / modGRF(1-29). They are all the same thing but with a different name. They come in vials ranging in material weights measured in milligrams (mg) consisting of a solid freeze-dried (lyophilized) substance.

4 – There are various types of GHRP’s. GHRP-6, GHRP-2, Hexarelin, and Imaporelin. The differences between them are potency and side effects. GHRP-6 is very potent and makes you quite hungry. GHRP-2 is potent and can slightly affect your sleep somewhat. Hexarelin is very potent but you can desensitize from higher dosages. Imaporelin is potent with the minimalist side effects of all 4 GHRP’s.

5 – Peptides are dosed via a regular 1mL needle syringe typical to what a diabetic would use. It is administered Subcutaneously (SubQ) (just under the skin into the fat tissue), most usually around the abdomen region.

6 – The required amount (saturation dose) is 1mcg (microgram) per Kg (Kilogram) of bodyweight. The typical usage and for ease of measuring is 100mcg of modGRF(1-29) and/or 100mcg of your choice of GHRP. Lower dosages will simply result in less GH release due to a slightly weaker GH pulse and reduce any side effects you may have. A higher dose will have minimal benefit and is more a waste of money than anything else. But, in saying that, the more frequently dosed in any given day would result in more frequent pulses.

7 – Mixing (reconstitution) the lyophilized product in their vials with Bacteriostatic Water (BW) can take some getting used to. The idea is not to add too much dilution. Typical rule of thumb is to add 0.5mL of BW to 1mg of Peptide. So a 2mg vial should reconstitute with 1mL BW. 5mg with 2.5mL, 10mg with 5mL, etc. Squirt the BW along the inside wall of the vial in a smooth controlled manner being cautious not to agitate the mixture too much. It will dissolve itself and become clear. You can roll the vial gently between your fingers or hands but don’t shake it to dissolve. The reconstitute is ok to be drawn once fully dissolved.

8 – On a 1mL needle, there are either 50 tick marks from 0-100, skipping every odd number OR 100 international units (IU). A 100mcg dose is half way between the 2nd and 3rd tick mark, OR 5 IU’s (if you followed the above reconstitution). There are no half tick marks. It is OK to draw modGRF and GHRP into the one needle for a single shot. It is NOT OK to mix peptides in the same vial or syringe for storage.

9 – Reconstituted peptide should be stored in the refrigerator to prevent degradation. Left at room temperature, peptide will degrade within days but kept in the fridge will last months. You can pre-load syringes and store in freezer if you want but it is more of a hassle than being worth the effort.

10 – Doses can be taken throughout the day but at no less than 3 hour intervals between doses. 1 dose a day is typical for light injury repair, anti-aging effects, deeper sleep, and better quality of life. The most beneficial would be to dose immediately prior to going to bed for your daily sleep period. Sleep is the time when our pituitary is most active. 2 or 3 doses per day will give the added benefit of lean tissue build, and fat loss, considering your diet consists of good quality foods.

11 – Doses should be taken on empty stomach to benefit the most. This is usually 3 hours or more.

12 – Do not consume food for between 15-30 minutes after your dosage. Best time is around 20-25 minute mark. GH pulses should peak within about 10 minutes after dosage. Fats and Carbohydrates affect the pulse dramatically. Protein has no effect on pulse and you can have a pure protein source in your stomach at anytime if you so choose to.

13 – Dosage timing can be beneficial to your goals. For muscle growth, the 2nd most beneficial time to dose is post workout (PWO). Best time is pre-bed because sleep is when we recover and our cells repair and grow. Within 30 minutes should be fine but sooner the better. Remember to have your meal 20-25 minutes after dose.

14 – For fat loss, your supplemental dose is 1 hour pre-cardio exercise after a long fasting without food. Best time is after waking up and before breakfast. During cardio exercise, maintain a moderate intensity for between 30-60 minutes. 45 minutes is a good session. You do not want to go too hard or too long. A moderate pace will utilize Free Fatty Acids (FFA) at the highest rate for energy. Refrain from eating for approximately 2 hours after your exercise because this is the time the body is still burning fat as fuel. You must eat throughout the day to reduce the chance of muscle catabolism (breakdown).

15 – These Peptides can be used on a daily basis for the rest of your life without any harm. Enjoy!!!

Written and Thanks goes to Aussie

Basic Guide for Peptides

Protein peptides profile

peptides are short polymers of amino acids linked by peptide bonds. They have the same peptide bonds as those in proteins, but are commonly shorter in length. The shortest peptides are dipeptides, consisting of two amino acids joined by a single peptide bond. There can also be tripeptides, tetrapeptides, pentapeptides, etc. peptides have an amino end and a carboxyl end, unless they are cyclic peptides. A polypeptide is a single linear chain of amino acids bonded together by peptide bonds. Protein molecules consist of one or more polypeptides put together typically in a biologically functional way and sometimes have non-peptide groups attached, which can be called prosthetic groups or cofactors.
Protein peptides are the preferred method for the body to absorb nitrogen into the muscles because the proteins can be absorbed intact. In fact, peptides are absorbed over 200 percent faster than free-form amino acids or whole protein molecules. The faster protein is absorbed in the body, the more it promotes protein synthesis ? a key component in muscle development. When the body breaks down proteins, it breaks them down into peptides, which in turn creates nitrogen in the bloodstream. Over 70 percent of nitrogen found in the bloodstream is in peptide form. Also, protein peptides made from whey are over 65 percent better at retaining nitrogen than regular whey. Other valuable characteristics of peptides are that it helps weight loss by stimulating the brain center that tells the body that it is full. peptides stimulate Insulin Growth Factors, which develop muscle tissue. peptides are also found to aid gastrointestinal and liver function.

MGF

MGF is a protein peptide that activates the process that repairs muscle damage. When muscles are worked out, or damaged, MGH triggers the metabolic agents in the body such as IGF-1 to repair and replace damaged muscles. Natural MGH declines with age and is a major cause of muscle tissue decreases as we grow older. MGF in clinical doses has shown remarkable results for not only muscle repair but new muscle growth as well.<

CJC-1295

CJC-1295 is a tetrapeptide that was developed to aid in weight loss. It is a growth hormone releasing hormone (GHRH) that has a longer half-life in the body than other GRHRs.

PT-141 (Bremelanotide)

PT-141 is a heptapeptide developed from Malanotan II. It has been studied for a variety of applications, including cosmetically as a sunless tanning agent and as a sexual dysfunction drug that could treat erectile dysfunction in men and arousal dysfunction in women. PT-141 was found to have some unwanted side effects on the circulatory system (high blood pressure) and further testing has been delayed.

Hexarelin

Hexarelin is a hexapeptide that is injected and stimulates the pituitary gland to produce growth hormone (GH). It also stimulates IGF-1 response, making it ideal for muscle mass and strength increases as well as fat loss. Because Hexarelin stimulates natural GH, it is often used after a cycle of HGH to avoid shutdown of natural GH production.

ALCAR (Acetyl-L-carnitine)

ALCAR is an experimental acetylating agent that modifies protein structures. It is being tested for its unique quality of improving brain function and as a possible cure to diseases such as Alzheimer’s and dementia.

IGF-DES

IGF DES is a potent Insulin Growth Factor peptide that is fast-acting and has the ability to act with IGF receptors even after they have become damaged by lactic acid during workouts. It also seems to have a longer half-life than its counterparts like IGF-1 or IGF-LR3.

Follistatin 344

Follistatin 344 is a peptide that inhibits myostatin, the chemical in the body that regulates muscle growth. When the body produces myostatin, it tells the muscles to stop growing, which is why inhibitors like Follistatin allow bodybuilders to grow larger muscles. Reports on this peptide vary and there are complaints that it adversely affects the tendons.

Triptorelin

Triptorelin (aka Decapeptyl, Diphereline, Gonapeptyl, Trelstar and Variopeptyl) is a decapeptide that was developed to help treat prostate cancer. It is in a class of drugs called gonadotropin-releasing hormone agonists (GnRH agonists). The result from taking Triptorelin is a gradual reduction of testosterone in the body, which is why the best use for this peptide is part of a post-cycle therapy where you need to reduce testosterone before it aromatizes into estrogen.

PEG-MGF (PEGylated Mechano Growth Factor)

PEG-MGF is a peptide hormone that increases the stem cell count in muscle tissue. Stem cells allow the muscle to heal and to grow in number and size. The PEG, or polyethylene glycol, is attached to the MGF to make the peptide last longer in the body by increasing its half-life. This allows the MGF molecule to act more consistently on the muscle tissues, providing greater results.

Melanotan II

Malanotan II is a synthetic analog of the body’s natural melanocortin peptide hormone ? the alpha-melanocyte stimulating hormone (a-MSH). It is being developed as a tanning agent for skin and as a drug to treat sexual dysfunction in both men and women.

GHRP-2 (Growth Hormone Releasing peptide-2)

GHRP-2 is a hexapeptide that acts on the pituitary gland to release Human Growth Hormone (HGH). This peptide has a strong anabolic effect and creates strong muscle gains and weight loss. Besides boosting HGH levels, it also has a strong effect in boosting IGF-1 levels. This peptide works best in conjunction with HGH because it stimulates the natural HGH levels making it less likely that synthetic HGH will shut down the pituitary gland?s natural production.

Ipamorelin

Ipamorelin is a pentapeptide that acts on the pituitary gland to produce Human Growth Hormone. An increase of HGH levels builds muscles and burns fat at incredible rates. Studies have shown that there is little long-term effect that Ipamorelin has on the pituitary gland’s ability to naturally produce growth hormones.

IGF-1 Long R3

IGF- 1 Long R3 s an insulin-growth factor peptide that increases amino acid transport to cells, increases glucose transport, increases protein synthesis, decreases protein degradation and improves RNA synthesis. Unlike regular IGF-1, the Long R3 version doesn’t easily bind to the IGF binding proteins that inhibit the biological actions of IGFs.

GHRP-6

Growth Hormone Releasing peptide-6 (GHRP-6) is an amino-acid peptide that triggers the body to release growth hormone. Growth Hormone burns fat and increases muscle strength and mass. GHRP-6 has the distinct characteristic of being a Ghrelin antagonist. Ghrelin is a chemical in the body that helps store fat, which makes GHRP-6 a great peptide to take to get lean.

HGH

Human Growth Hormone (HGH) is a synthetic version of the natural growth hormones produced by the pituitary gland. Growth hormones tell the body to build muscle and burn fat in people during their puberty years. Growth hormone production slows as we age and is a major cause of how the body looses strength in old age. There are many types of HGH on the market today and is available by prescription and on the black market.

HGH Fragment 176-191

HGH Fragment 176-191 is a piece of the Growth Hormone chain of amino acids ? the part of the chain from amino acid number 176 through the amino acid number 191. It is believed by the developers of this peptide that the fragment of amino acids is responsible for the fat burning properties of HGH. They were attempting to isolate a stronger formula that targeted only fat burning to market as a weight loss drug.

Adapotide

Adapotide is a new research drug used to treat cancer but has shown remarkable results for weight loss. It is in a class of drugs called angiogenesis inhibitors that work to block blood flow to various parts of the body, in this case, fat cells. Clinical trials involving primates have shown remarkable results in cutting belly fat and overall weight loss.

Please feel free to add any peptides not discussed on this post. Ive been off the boards for a while. I have to admit, It feel good to speak about peptides again. I used to work for 2 major companies in this industry, I wasn’t able to discuss usage, dose recommendations, or what times of the day are better for certain IGF family members, including the MGF family, as well as the ghrp, and ghrh family. When You are a sponsor, you really have to watch what you say. If I were ever to give advise online in regards to dosing or protocols, I would have broken my own disclaimer! Its been about a year since Ive been on this board. Its nice to be back. Lets get the discussions going!!!

Stylus187